Rapid and sensitive detection of nucleic acids and proteins
is vital for the identification of pathogenic agents. Biomolecules, such as
F1-ATPase, have been previously shown to provide a single molecule detection
system for DNA using a detection signal that is visible by fluorescence
microscopy (US 6,989,235).
Prof. Wayne Frasch at Arizona State University has developed
a detection device for the identification of targets that employs and further
develops the immobilized F1-ATPase. Target detection is achieved when the
metabolite of interest binds to a protein attached to the F1-ATPase and is
subsequently bound by a detection probe which binds only in the presence of the
specific metabolite.
The device provides an extremely sensitive technique for
target detection and has a wide range of applications including clinical
diagnostics, forensic analysis, gene expression analysis, DNA sequencing, DNA
proofing, and DNA computing.
Potential Applications
- Nanodetection devices
- Clinical diagnostics
- Forensic analysis
- Gene expression analysis
- DNA sequencing
Benefits and Advantage
- Multiple ligation sites for increased specificity
- Does not rely upon hybridization alone for specificity
- Hybridization can occur in solution, decreasing detection
time
- Decreases Error rates from 0.2% down to
0.0001%
Download Original PDF
For more information about the inventor(s) and their
research, please see
Dr.
Frasch's departmental webpage
