Next-generation DNA sequencing has been developed to
improve the classical Sanger sequencing process. Common to many next-generation
sequencing approaches is a problem of limited read length due to the steady loss
of DNA from an array surface and the growth of out-of-phase signals. Therefore,
there is a need for greater stabilization of DNA attachment to the surface and a
minimization of out-of-phase signal growth.
Researchers at Arizona State University have developed a
method for preparing highly stable, silane-derivatized glass surfaces for use in
DNA sequencing applications. By using heat treatment along with linker
molecules, the process removes loosely bound reactive groups that could
otherwise be dislodged, adversely affecting the accuracy of the sequencing and
producing errors.
Elevated temperature treatment in an aqueous environment
hydrolyzes the siloxanses where they bind to the glass surface. Thus, further
stabilization of the surface can be specified to reform these bonds by an
additional dry heat treatment.
Potential Applications
- Glass surface treatment for DNA sequencing
Benefits and Advantages
- Improved read length
- Increased surface stability
- Greater accuracy
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For more information about the inventor(s) and their
research, please see
Dr.
Williams' departmental webpage
