Infections caused by the Hepatitis C virus (HCV) are very
common worldwide (afflicting up to 3% of the population), and chronic infection
may develop into cirrhosis and liver cancer. For this reason a vaccine against
HCV would have great utility, with the HCV envelope protein 2 (E2) a promising
candidate because it can directly bind to a human cell receptor and plays a role
in viral entry. However, studies of producing E2 for a vaccine have shown that
much of the E2 produced is misfolded and thus not functional.
Researchers at the Biodesign Institute of Arizona State
University have developed a method to transiently overexpress two molecular
chaperones in the endoplasmic reticulum of plants in order to facilitate E2
folding and production. Western blot analysis showed that calnexin and
calreticulin overexpression in Nicotiana benthamiana significantly increased the
yield of properly-folded E2 compared to controls.
This discovery has the potential to accelerate the research for
a commercially-viable vaccine for Hepatitis C virus.
Potential Applications
- therapeutic for Hepatitis C virus
- prophylactic vaccine for Hepatitis C virus
Benefits and Advantages
- increases the yield of the promising vaccine candidate E2
- amenable to use in a plant system, with the attendant
advantages of greater safety, lower cost and easier scalability to industrial
production
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research, please see
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Mason's directory webpage
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Mason's departmental webpage