Many enzyme based applications and processes could be
improved if there were a means to increase or decrease the activity of a
specific enzyme under a particular set of conditions. In particular, being able
to inhibit one enzyme in the presence of others, stabilizing and maintaining its
catalytic activity over time, or enhancing its activity under nonnatural
conditions (high temperature, low pH, etc.) could greatly increase the utility
of enzymes in a variety of applications. Finally, the ability of switch enzymes
on and off depending on cooperative interactions between ligands (chemical logic
gating), could be very beneficial in the control of complex enzyme systems.
Researchers at Arizona State University’s Biodesign
Institute have developed proprietary methods to screen for molecules that
modulate enzyme activity, stability, either linearly or cooperatively. Based on
microarray technologies, the methods feature highly flexible experimental
design, rapid high throughput analysis of molecules and the ability to identify
multiple binders to a particular ligand.
Potential Applications
- These methods have various applications in the consumer
products, biotechnology, pharmaceutical and medical diagnostic industry for:
- Decreasing amounts of enzymes needed
- Extending the range of enzymatic conditions
- Stabilizing enzymes over time/temperature
- Modulating enzyme activity
Benefits and Advantages
- The present invention facilitates:
- Rapid high throughput enzyme modification
- Identification of multiple binders in situ
- Modulation of enzymatic activity under desired
conditions
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For more information about the inventor(s) and their
research, please see
Dr.
Woodbury's departmental webpage
Dr.
Woodbury's directory webpage
Dr.
Johnston's directory webpage
